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The main difference between androgenic and anabolic is that androgenic steroids generate male sex hormone-related activity whereas anabolic steroids increase both muscle mass and the bone mass(i.e. they activate the estrogen receptor). The increase in the bone's density is primarily due to increased bone mass because the increased estrogen levels suppress bone formation. The increase in protein synthesis, however, is mainly due to increased bone mineral content, and in fact some study authors have shown that androgenic steroids might increase bone mass even in the presence of low protein intake (Boechler 2005). Although muscle protein synthesizing enzymes in muscle is not stimulated by androgens but by androgens and androgens stimulate muscle protein breakdown. There are at least two ways of looking at this because both are based on the fact that the androgens are anabolic. For example, although a man with high testosterone may be anabolic to anabolic steroid users, he will also be hyper- androgenic to non-anabolic steroid users at the same time. An example of hyper- androgenic muscle is an athlete with anabolic steroids. Both anabolic and non-anabolic steroids stimulate muscle protein synthesis. A more recent scientific investigation of muscle protein synthesis is conducted in humans for the first time in 2002 by David Haan et al. (Haan 2004). The researchers, who were not blinded to the subjects' testosterone status, studied male subjects in their mid-twenties. They measured the expression of four mRNA and protein-coding genes known to be stimulated in muscle cells. The first was the gene, PGC-1α, that stimulates glycogen synthesis (or glycogenolysis) of myofibrils. The expression of other genes (for example, PGC-1α, myokinase 1, MAPK, etc.) had no correlation with the stimulation of muscle protein synthesis by testosterone. The data showed that there was, in fact, no correlation between the mRNA expression of PGC1α and the stimulation of muscle protein synthesis by testosterone in muscle cells. In addition, the study found that serum free testosterone levels were not correlated with muscle protein synthesis in muscle. (Haan et al. 2002: 10) This means that muscle protein synthesis does not depend on testosterone. This means that any muscle hypertrophy caused by androgens could not be attributed to testosterone stimulation, and the study's conclusion that testosterone was not necessary to induce muscle hypertrophy was correct. The second study that investigated muscle protein expression of four genes that stimulate protein synthesis in muscle was performed by the same group of researchers in 2005. (Shaw et al. 2005) After performing Similar articles: